Mass Cytometry of the Human Mucosal Immune System Identifies Tissue- and Disease-Associated Immune Subsets

Vincent van Unen, Na Li, Ilse Molendijk, Temurhan Mine, Thomas Höllt, Andrea E van der Meulen-de Jong, Hein W Verspaget, M Luisa Mearin, Chris J Mulder, Jeroen van Bergen, Boudewijn P.F. Lelieveldt, Frits Koning
Immunity, Volume 44, Number 5, page 1227--1239 - may 2016
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Inflammatory intestinal diseases are characterized by abnormal immune responses and affect distinct locations of the gastrointestinal tract. Although the role of several immune subsets in driving intestinal pathology has been studied, a system-wide approach that simultaneously interrogates all major lineages on a single-cell basis is lacking. We used high-dimensional mass cytometry to generate a system-wide view of the human mucosal immune system in health and disease. We distinguished 142 immune subsets and through computational applications found distinct immune subsets in peripheral blood mononuclear cells and intestinal biopsies that distinguished patients from controls. In addition, mucosal lymphoid malignancies were readily detected as well as precursors from which these likely derived. These findings indicate that an integrated high-dimensional analysis of the entire immune system can identify immune subsets associated with the pathogenesis of complex intestinal disorders. This might have implications for diagnostic procedures, immune-monitoring, and treatment of intestinal diseases and mucosal malignancies.

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BibTex references

@Article { VLMMHVVMMVLK16,
  author       = "Unen, Vincent van and Li, Na and Molendijk, Ilse and Mine, Temurhan and H\öllt, Thomas and Meulen-de Jong,
                  Andrea E van der and Verspaget, Hein W and Mearin, M Luisa and Mulder, Chris J and Bergen, Jeroen van and
                  Lelieveldt, Boudewijn P.F. and Koning, Frits",
  title        = "Mass Cytometry of the Human Mucosal Immune System Identifies Tissue- and Disease-Associated Immune Subsets",
  journal      = "Immunity",
  number       = "5",
  volume       = "44",
  pages        = "1227--1239",
  month        = "may",
  year         = "2016",
  url          = ""

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» Vincent van Unen
» Na Li
» Thomas Höllt
» Jeroen van Bergen
» Boudewijn P.F. Lelieveldt
» Frits Koning