![]() | Evolution of cellular diversity in primary motor cortex of human, marmoset monkey, and mouse |
The primary motor cortex (M1) is essential for voluntary fine motor control and is functionally conserved across mammals. Using high-throughput transcriptomic and epigenomic profiling of over 450,000 single nuclei in human, marmoset monkey, and mouse, we demonstrate a broadly conserved cellular makeup of this region, whose similarity mirrors evolutionary distance and is consistent between the transcriptome and epigenome. The core conserved molecular identity of neuronal and non-neuronal types allowed the generation of a cross-species consensus cell type classification and inference of conserved cell type properties across species. Despite overall conservation, many species specializations were apparent, including differences in cell type proportions, gene expression, DNA methylation, and chromatin state. Few cell type marker genes were conserved across species, providing a short list of candidate genes and regulatory mechanisms responsible for conserved features of homologous cell types, such as the GABAergic chandelier cells. This consensus transcriptomic classification allowed the Patch-seq identification of layer 5 (L5) corticospinal Betz cells in non-human primate and human and characterization of their highly specialized physiology and anatomy. These findings highlight the robust molecular underpinnings of cell type diversity in M1 across mammals and point to the genes and regulatory pathways responsible for the functional identity of cell types and their species-specific adaptations.
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@Article { BJHLTKCHKSEYAABBCCCDDDDDDDFFGGGHHKKLLLLLMMMMNOOPPPPRRRRSSSSTTTWXYZACCDGHHHHKKKLLMPRRSSSWKHTMMTZZFELL20b, author = "Bakken, Trygve and Jorstad, Nicholas L. and Hu, Qiwen and Lake, Blue B. and Tian, Wei and Kalmbach, Brian E. and Crow, Megan and Hodge, Rebecca and Krienen, Fenna M. and Sorensen, Staci A. and Eggermont, Jeroen and Yao, Zizhen and Aevermann, Brian and Aldridge, Andrew I. and Bartlett, Anna and Bertagnolli, Darren and Casper, Tamara and Castanon, Rosa G. and Crichton, Kirsten and Daigle, Tanya L. and Dalley, Rachel and Dee, Nick and Dembrow, Nikolai and Diep, Dinh and Ding, Song-Lin and Dong, Weixiu and Fang, Rongxin and Fischer, Stephan and Goldman, Melissa and Goldy, Jeff and Graybuck, Lucas and Herb, Brian R. and Hou, Xiaomeng and Kancherla, Jayaram and Kroll, Matthew and Lathia, Kanan and Lew, Baldur van and Li, Yang Eric and Liu, Christine S. and Liu, Hanqing and Mahurkar, Anup and McMillen, Delissa and Miller, Jeremy and Moussa, Marmar and Nery, Joseph R. and Orvis, Joshua and Owen, Scott and Palmer, Carter R. and Pham, Thanh and Plongthongkum, Nongluk and Poirion, Olivier and Reed, Nora M. and Rimorin, Christine and Rivkin, Angeline and Romanow, William J. and Sedeño-Cortés, Adriana E. and Siletti, Kimberly and Somasundaram, Saroja and Sulc, Josef and Tieu, Michael and Torkelson, Amy and Tung, Herman and Wang, Xinxin and Xie, Fangming and Yanny, Anna Marie and Zhang, Renee and Ament, Seth A. and Corrada Bravo, Hector and Chun, Jerold and Dobin, Alexander and Gillis, Jesse and Hertzano, Ronna and Hof, Patrick R. and H\öllt, Thomas and Horwitz, Gregory D. and Keene, Dirk and Kharchenko, Peter V. and Ko, Andrew and Lelieveldt, Boudewijn P.F. and Luo, Chongyuan and Mukamel, Eran A. and Preissl, Sebastian and Regev, Aviv and Ren, Bing and Scheuermann, Richard and Smith, Kimberly and Spain, William J. and White, Owen R. and Koch, Christof and Hawrylycz, Michael and Tasic, Bosiljka and Macosko, Evan Z. and McCarroll, Steven A. and Ting, Jonathan T. and Zeng, Hongkui and Zhang, Kun and Feng, Guoping and Ecker, Joseph R. and Linnarsson, Sten and Lein, Ed", title = "Evolution of cellular diversity in primary motor cortex of human, marmoset monkey, and mouse", journal = "BioRxiv", month = "April", year = "2020", note = "preprint", url = "http://graphics.tudelft.nl/Publications-new/2020/BJHLTKCHKSEYAABBCCCDDDDDDDFFGGGHHKKLLLLLMMMMNOOPPPPRRRRSSSSTTTWXYZACCDGHHHHKKKLLMPRRSSSWKHTMMTZZFELL20b" }
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