CyTOFmerge: Integrating mass cytometry data across multiple panels

Tamim Abdelaal, Thomas Höllt, Vincent van Unen, Boudewijn P.F. Lelieveldt, Frits Koning, Marcel Reinders, Ahmed Mahfouz
Bioinformatics, Volume btz180 - 2019
Download the publication : 2019_cytofmerge.pdf [953Ko]   2019_cytofmerge_supplemental.pdf [28Mo]  
High-dimensional mass cytometry (CyTOF) allows the simultaneous measurement of multiple cellular markers at single cell level, providing a comprehensive view of cell compositions. However, the power of CyTOF to explore the full heterogeneity of a biological sample at the single cell level is currently limited by the number of markers measured simultaneously on a single panel. To extend the number of markers per cell, we propose an in silico method to integrate CyTOF datasets measured using multiple panels that share a set of markers. Additionally, we present an approach to select the most informative markers from an existing CyTOF dataset to be used as a shared marker set between panels. We demonstrate the feasibility of our methods by evaluating the quality of clustering and neighborhood preservation of the integrated dataset, on two public CyTOF datasets. We illustrate that by computationally extending the number of markers we can further untangle the heterogeneity of mass cytometry data, including rare cell population detection.

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BibTex references

@Article { AHVLKRM19,
  author       = "Abdelaal, Tamim and H\öllt, Thomas and Unen, Vincent van and Lelieveldt, Boudewijn P.F. and Koning, Frits
                  and Reinders, Marcel and Mahfouz, Ahmed",
  title        = "CyTOFmerge: Integrating mass cytometry data across multiple panels",
  journal      = "Bioinformatics",
  volume       = "btz180",
  year         = "2019",
  note         = "doi: 10.1093/bioinformatics/btz180",
  url          = ""

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» Tamim Abdelaal
» Thomas Höllt
» Vincent van Unen
» Boudewijn P.F. Lelieveldt
» Frits Koning
» Marcel Reinders
» Ahmed Mahfouz